Development of approaches for the bioconversion of dietary fibers in flax meal using cellulolytic enzymes

Abstract

The object of the study is the process of enzymatic hydroly sis of dietary fiber in flax meal, aimed at the bioconversion of cel lulose into soluble sugars. The work considers the use of cellu lolytic enzymes and an antioxi dant for the hydrolysis of cellu lose in flax meal. This allows to increase the bioavailability of sol uble sugars and other nutrition al compounds, as well as to pre serve polyunsaturated fatty acids (PUFA) of the lipid component of the meal, in particular alpha-lin olenic acid (ALA), from oxidative destruction. The rational param eters of the enzymatic hydroly sis process are determined: pH 4.5 and temperature 50 °C, which ensure the maximum yield of sol uble sugars (11.4 %) with mini mal losses of ALA (9.0 %). The use of an antioxidant – sodium salt of erythorbic acid (E 316) to pro tect PUFA from oxidative dam age during enzymatic hydrolysis is also considered. The most effec tive concentration of sodium ery thorbate was 0.03–0.035 %, which minimizes ALA losses to 1.4 %. The results of the research are important for the development of new technologies for process ing flaxseed meal, which will con tribute to improving the quality of products and their preserva tion for a long period. The results obtained are explained by bio chemical and chemical interac tions between the components of the reaction mixture (enzyme, buffer, antioxidant) and the com ponents of flaxseed meal (dietary fiber, lipid complexes). This leads to an increase in the efficiency of cellulose hydrolysis and the pres ervation of PUFA from oxidation. The results obtained allow to con sider flaxseed meal with hydro lyzed cellulose as a promising product for the food industry and feed production