Експериментальне обґрунтування біопсії сім'яників

Abstract

У досліді на статевозрілих бугайцях 6-9-місячного віку експериментально обгрунтовано можливість виконання біопсії сім'яників за допомогою запропонованого приладу, що дає можливість своєчасно виявити і оцінити стан сперматогенезу, клітин сперматогеного епітелію та інтерстиційної тканини сім'яника.

В опыте нa половозрелых быках 6-9-месячного возраста экспериментально обоснована возможность выполнения биопсии семенников с помощью предложенного устройства, что позволяет своевременно определить и оценить состояние сперматогенеза, клеток сперматогеного эпителия и интерстициальной ткани семенника.

Biopsy tissue of organs as a way of diagnosis of diseases, that indentified by histological changes and histochemical and biochemical exploration, widely used in medical and veterinary clinical practice and scientific research. At the first time in veterinary andrology for biopsy of parenchyma of testis by bulls and others farm animals developed and patented the device, that gives the opportunity to obtain sufficient material for any exploration: biochemical, histological, histochemical and contact preparation from tissues etc. The most important meaning of biopsy among other diagnostic manipulations are that differentiated obstructive infertility (aspermia) for obstruction testicles channels by physiologically formed germ cells and non-obstructive infertility, when spermatogenesis is disrupted for expressed dysfunction of testicles. Method that we have proposed is needle biopsy refers to TESA, that means, surgical intervention which means, specially developed device, that help to get a sample of tissue parenchyma. In experiments were used calves in age 6-9 months, which belonged to МОС , Ukraine“ ZNAU апа training clinic for big animals the faculty of veterinary medicine . Scheme of the experiment included: the definition of the general condition of the animals, the processing operating place, anesthesia testicles, skin puncture, putting the stylet device to the appropriate depth in the parenchyma of the testis, scroll clockwise 180-360°, pulling the stylet, biopsy extraction, processing wounds. The resulting parenchyma testis biopsy cylindrical shape of varying length, that depending on the depth of the introduction of a stylet testes. The weight pale pink parenchyma, elastic, compact. We have established that biopsy of bulls testicles did not affect the overall performance of their physiological state, as the temperatures, pulse, respiration varied physiological limits. Locally volume scrotum and testes unchanged during the period of observation, which lasted 6 months. Stab wound healed without any clinical signs, indicating the progress of inflammation. As result by the most of testicles channels established by calves in the age of 6-9 months showed, together with spermatohony, epermatopm 1st and 2nd order and spermatids, on the sharp stages of the formation of sperm. In the testicles channels by bulls the spermatogenesis is uneven in some channels it is well manifested, but in others - weak. So, testicular biopsies using the proposed device and method makes it possible to detect and assess the state of spermatogenesis, cells of spermatogenic epithelium and interstitial testis tissue.