Рентгенденситометрія стегнових кісток білих щурів після введення мезенхімальних стовбурових клітин за експерементального гіпотиреозу

Abstract

В статті висвітлено зміни кісткової щільності, а саме, зменшення мінеральної щільності стегнових кісток щурів із гіпотиреоїдною функцією за допомогою рентгенденситометрії. Метою проведеного дослідження було вивчення впливу стовбурових клітин після введення на мінеральну щільність стегнових кісток білим щурам за експериментального гіпотиреозу, яке характерне при тиреоідній недостатності в тварин.

В статье освещены изменения костной плотности, а именно, уменьшение минеральной плотности бедренньх костей крыс с гіпотиреоїдною функцией с помощью рентгенденситометрії. Целью проведенного исследования было изучение влияния стволовых клеток после введения на минеральную плотность бедренных костей, белым крысам за экспериментального гипотиреоза, которое характерно при тиреоїдній недостаточности у животных.

The article highlights the changes in the bone density, namely, the reduction of mineral density of the femur of rats with Hotineanu function with rentenanstalt. The aim of this study was to investigate the effect of stem cells after injection in mineral density of femoral bones of white rats in experimental hypothyroidism, which is when Tirion failure in animals. In our time, relevant is the study of diseases of the thyroid gland in connection with the distribution of thyroid insufficiency in animals. The most common form of the disease in animals is low hypothyroid function. Diseases of the thyroid gland are numerous and each of them has its own clinical picture, the symptoms. Moreover, the same symptom can manifest the opposite way. In connection with the weakening of attention to the prevention of endemic goitre in recent years there has been a tendency to spread, which can have negative health consequences for both small and large animals. The correctness of the diagnosis, treatment and prevention of iodine deficiency States will prevent. Recent publications regarding diseases of the thyroid gland, it is known that when there is insufficient amount of thyroid hormones in animals, may experience a decrease in bone density with various pathological conditions. Which lead to osteoporosis a multifactorial nature, frequent disability of animals, most often this pathology is common in purebred animals, which later can result in death as a result of fractures of the proximal femur. Leads to an increase of calcium in blood serum, which kompensiruet through leaching of calcium from bones, teeth, spine, tail of the animal. This pathology will depend on the complexity and duration of thyroid deficiency, and full or partial disruption of hormonal functions in the body. This is because in hypothyroidism, the metabolic rate falls, violated all metabolic processes in the animal body that leads, as a complication of disruption of the liver, kidney, heart, pancreas, which is typical in complex forms hotiron dysfunction. The experiments were performed on white rats at the age of 1.5 months, with an average initial body weight of 195+3 Rats were divided into five groups: the first group of animals, which were injected isotonic solution instead of mesenchymal stem cells, the second group MSCS were injected into the cavity of the heart, the third group was injected MSCS in the thyroid gland, and the fourth was introduced in MSCS intravenously, and the fifth group conventional treatment(thyroxine). Each animal through various routes of administration, which are given in the experimental table was put at 2 million. mesenchymal stem cells in sterile conditions. All animals before the introduction mesenchymal stem cells (MSCS) within 65 days of the experiment instead of drinking water vidouville 1% solution of potassium perchlorate. As feed rats received a balanced complete feed designed for feeding this species. The research was carried out in the Helsinki Declaration of the world medical Association on ethical principles for scientific and veterinary research. involving animal and obtained results were processed statistically using student's criterion t °.