Морфологічні та біохімічні показники крові бородатих агам, уражених Oxyuris thelandros

Abstract

У статті наведені морфологічні та біохімічні показники крові бородатих агам, уражених оксіурозм. У морфологічних показниках крові спостерігається збільшення кількості лейкоцитів на 33,3 %, еозинофілія на 100 %, гетерофілія – на 51,8 %, азурофілія – на 70,4 %, та зменшення базофілів – на 67,9 %, лімфоцитів – на 8,4 %, моноцитів – на 66,7 %, що пов’язано з адаптацією організму до паразитування оксіур. В біохімічних показниках збільшення глобулінів на 56,3 %, зменшення альбумінів на 45,2 % та зростання активності ферментів АлАТ та АсАТ відбувається в результаті патогенної дії оксіур на організм рептилій.

The study of the morphological and biochemical parameters of the blood of reptiles is of great importance, both for the development of hematology of reptiles, and for veterinary herpetology as a whole. The research was conducted in the "Afalina" Zoo, Mykolaiv. Two groups of reptiles (n = 10) - control non-invasive (clinically healthy) and experimental - reptiles, affected by Oxiuris thelandros were formed. In the blood of reptiles infected with oxyurons, a 13.6% reduction in hemoglobin was observed to 67.2 ± 0.6 g /l against 77.8 ± 0.5 g/l in control due to a significant reduction of erythrocytes by 49.6% relative to the control groups. However, the number of leukocytes increased by 33.3% from the indicator of 9.9 ± 0.6 g/l in the control to 13.2 ± 0.5 g/l in the experimental group, indicating the manifestation of the protective reaction of the organism. The leukogram in the blood of bearded dragon in the experimental group was characterized by eosinophilia with an over-control of 100%, as well as by 51.8% heterophilia and 70.4% azurophyllium. It should be noted that in the leukogram of the experimental group of reptiles, the number of basophils decreased significantly by 67.9%. The number of lymphocytes in the experimental and control group of reptiles varied slightly from 69.3 ± 0.8% to 63.5 ± 0.6%, that is, there was a decrease by 8.4%. Also significantly decreased the number of monocytes from 0.4 ± 0.1% to 1.2 ± 0.1% in the control, indicating the immunodeficiency state of the body of diseased animals. Thus, for oxyurase of bearded dragon in morphological parameters of blood, an increase in the number of leukocytes, eosinophilia, heterophilia, azurophyllia and a decrease in basophils, lymphocytes, and monocytes is observed, which is due to the adaptation of the organism to the parasitic oxyur. With the course of invasion in bearded dragon, there was a significant decrease in albumin content by 45.2% from 3.1 ± 0.6 g/cm3 in control to 1.7 ± 0.2 g/cm3 in the experimental group. Against the background of reducing the number of albumins, the number of globulins increased by 56.3% from 3.2 ± 0.5 g/cm3 in control to 5.0 ± 0.4 g/cm3 in the experimental. Such oscillations of albumins and globulins affected the total protein, which in the experiment was 6.7 ± 1.1 g/cm3, and in the control 6.3 ± 0.4 g/cm3. The increase was only 6.3%. However, the ratio of albumins to globulins influenced the formation of A/G coefficient. In the experimental group, the indicator was 0.3 versus 1.0 to control. In invasive reptiles, an increase in the activity of the enzymes AlAT and AsAT was observed at 83.2% and 86.6%, respectively, from 11.9 ± 0.2 un/l and 17.2 ± 0.6 un/l in the control to 21.8 ± 1.0 un /l and 32.1 ± 1.1 un/l in the experimental group. The indicated changes in the activity of enzymes confirm the development of the pathological process in the liver parenchyma bearded dragon, as well as the occurrence of concomitant structural and functional changes in other internal organs. Consequently, for oxyurase of bearded dragon, important links in the pathogenesis are the imbalance in the metabolism of proteins and enzymes, allergy to the organism.