Визначення параметрів гострої токсичності кормової добавки «Біостим 40» для щурів

Abstract

Дослідженнями встановлено, що доза кормової добавки «Біостим 40» у 30000 мг/кг маси тіла білих щурів, за внутрішньошлункового введення, не викликає клінічних ознако отруєння і загибелі тварин і тому, згідно класифікацією хімічних речовин за ступенем небезпечності (ГОСТ 12.1.007 – 76), її можна віднести до 4 класу токсичності – малотоксичні речовини.

Domestic animals have a positive influence on economic and physical human health because the contact with them increases physical activity and decreases arterial pressure, the risk of hypertensive people to have cardiovascular diseases, stress rate, frequency of depressions and increases the level of self-estimation as well as social activity of children and teens. Nowadays numerous commercial pet foods for domestic animals are developed with exaggeration of minimal recommendations that can cause eating disorders, metabolic disorders and animals’ obesity, thus, to ensure the protection for animals it is important to pay attention to pharmacological and toxicological analyses of food additives presenting necessary recommendations of their usage. To determine the acute toxicity of high-energetic protein, vitamin and mineral food additive “Biostim 40” there has been carried out a research on grown-up males of white rats with a mass in 200-230 g. Animals have been divided into 7 groups of 8 rats in every (n=56). Food additive has been fed individually to every rat in doses of 2000, 4000, 6000, 10000, 20000 and 30000 mg/kg per body mass. There have been implemented observations of rats’ general state, behavior, their orientation, reflexes of hearing and vision, eating and drinking, state of their fur and visible mucous membranes, breathing and excrements – all those factors have shown that even the highest doses of “Biostim 40” (30000 mg/kg per body mass), being inserted internally through the abdomen, have not caused any clinical cases of poisoning, rats’ deaths and, in accordance with international classification of chemical substances, “Biostim 40” may be enlisted to the 4th level of toxicity – the substance of low toxicity. Analyzing the dynamic of rats’ changing their body mass within 2 weeks it is possible to point out that one-time insertion of high doses of “Biostim 40” has assisted to increase rats’ body mass in 14-33% (р≤0.05), with the help of what we can say that this food additive is effective and safe stimulator of height and development of animals.