Аналіз штамів парвовірусу собак на основі послідовностей VP-2 гену

Abstract

В роботі проведено аналіз вакцин, що зареєстровані в Україні, досліджено поліморфізм VP2 гену вакцинних та патогенних штамів. Встановлено наявність SNP у послідовності VP2 гену, що дозволило провести філогенетичний аналіз дослідних штамів.

One of the most dangerous diseases of dogs is canine parvovirus enteritis. The causative agent of enteritis is Canine Parvovirus type 2 (CPV-2) of the subgroup Feline panleukopenia virus (FPV) of the species Carnivore protoparvovirus 1 of the family Parvoviridae. For prevention both in the world and in Ukraine vaccination is used. Despite this measure, parvovirus enteritis still causes death of dogs with a clinical and pathological pattern characteristic of the disease. The sequence of dog parvovirus strains was analyzed, which are presented in the GenBank database: No. MH213140.1, KP881653.1, GU212792.1, GU212790.1, FJ011098.1, GU212791.1, MG264079.1, GU392239.1, KF539801.1, MH491947.1, MH491855.1, DQ025952.1, MH329287.1, AY262281.1, KY921606.1. Among the given strains, 5 (GU212792.1, GU212790.1, FJ011098.1, GU212791.1, KY921606.1) are used for the manufacture of vaccines registered in Ukraine. Pathogenic isolates were isolated in Italy, Hungary, France, Taiwan, China, South Korea, etc. As a result of the studies, SNPs (Single Nucleotide Polymorphism) were revealed in the sequences and studied, and a phylogenetic tree was constructed (method of attachment of neighbors). The smallest distances were established between the vaccine strains VAC_M primodog (GU212790.1), VAC_P vanguard (GU212791.1), VAC_C quantum (GU212792.1). Intervet vaccine strains (FJ011098.1) and MX-VACVBC / 17 (KY921606.1) are in a separate cluster from the other three vaccine strains. They form a separate cluster. Also, the closest distances are established between the isolates according to the territorial distribution. The results obtained may indicate differences in the antigenic structure of circulating pathogenic strains of dog parvovirus. The lack of data from epizootological monitoring of the disease using molecular genetic methods in Ukraine shows the need for such studies.