Вплив температури на літичну активність бактеріофагу Phage SAvB14, специфічного щодо Staphylococcus aureus variant bovis

Abstract

В статті наведено результати дослідження впливу температури на літичну активність бактеріофагу Phage SAvB14, специфічного щодо Staphylococcus aureus variant bovis. Встановлено, що бактеріофаг Phage SAvB14 частково втрачає свою активність за температури вище 45 °C, що є важливим показником при виготовленні фагового препарату. Найбільш оптимальною температурою для зберігання фагу Phage SAvB14 є 4 - 8 °С.

Therapeutic effect in case of phagotherapy is determined by the degree of lytic activity of bacteriophages in relation to contagions. Various factors influence the lytic activity of phages including temperature, pH, shelf-life, etc. Temperature plays a considerable role in attachment, penetration, reproduction and duration of latent period. The purpose of this research was to learn the influence of different temperature on lytic activity of bacteriophage Phage SAvB14 which is active in relation to Staphylococcus aureus variant bovis. For the increase of bacteriophage titre Phage SAvB14 the strain of gold Staphylococcus aureus var. bovis 1491f (Certificate for the strain - #736, from 05.03.2019) was used which was abstracted from the sample of the secretion of mammary gland of the patient with subclinical form of mastitis. For determination of temperature influence (0, 4, 8 and 45, 55, 65 °C) aliquots were sown by a double agar in definite intervals by the generally accepted methods. The cups were incubated at temperature 37 °C during 18-24 hours. Research was repeated three times. According to the results of research it was determined that warming up of test tubes with bacteriophages at temperature 45 ºС reduced the activity of phages in 30 minutes of influence in 1,7 times. Temperature 55 and 65 ºС had more harmful effect, during the influence for the first 30 minutes the amount of active phages diminished in 2,5 and 5,3 times, and for 60 minutes destroyed the bacteriophage Phage SAvB14 by 89,9 and 93,3% respectively. The obtained results testify the thermolability of Phage SAvB14 which is an important factor in making phage preparation. The most optimum terms for storage of Phage SAvB14 are temperature regime within the limits of 4 and 8 °C. In case of such temperature lytic activity diminished only in 1,5 – 1,7 times, while in case of 0 °C in 2,4 times compared to the initial amount of bacteriophages.