Вплив Дерозалу на обмін азотистих та фосфорних сполук у печінці і м’язовій тканині курей за умов хронічного експерименту

Abstract

Дослідженнями встановлено, що додавання карбендазиму до корму дещо гальмувало засвоєння поживних речовин корма, що характеризувалось зниженням на 30 і 60 добу досліджень приросту маси тіла, в середньому, на 10 %, зменшенням вмісту білкового і небілкового азоту у печінці і м’язах курей усіх груп, підвищенням концентрації фосфору ДНК і РНК, вмісту кислоторозчинних фосфатів, фосфору фосфоліпідів і ліпоїдного фосфору, що свідчить про незначне гальмування синтезу білка, ДНК та РНК в печінці і м’язах птиці, а також про стимулювання синтезу ефірів фосфорної кислоти і реакції гліколізу за додавання пестициду.

Pesticides are toxic chemicals which are widely used as an effective means against pests and plant diseases as well as for animals’ protection against ectoparasites. They are also used to control rodents which transmit infectious diseases to people and animals (encephalitis, malaria, leptospirosis, rabies, listeriosis, etc.).

Biosynthesis of protein in tissues depends on many circumstances and, first of all, on the complete set of amino acids, the state of enzymes’ system and on the presence of stimulators and inhibitors of synthesis reaction. Xenobiotics including benzimidazole derivatives are a part of these compositions.

In Ukraine there are more than 20 medicines based on carbendazim. Thus, the analysis of the its influence on protein metabolism is relevant.

In the research the hens breed Rhode Island of the 38th line for egg and meat of 30 days of age, with the mass 1000-1200 gr. was used. They were divided into 2 groups - test (n=36) and 1 control (n=18). Нens were equally cared and fed with two food allowances: the first part up to 40 days of age and the second part from 40 days of age up to the end of the experiment (60 days). Every day throughout 60 days hens of the first and second testing groups received feed with the medicine Derosal (BAYER, Germany) in the form of 50 % aqueous suspension in dose of 90 and 900 mg. per 1 kg of body mass (0.01 and 0.1 LD50 for hens) accordingly. The control group of hens was fed without addition of the medicine. The research was carried out on the 30th and 60th day.

During the experiment it was found that addition of carbendazim to fodder slowed down the use of its nutrients. This situation was characterized by the fact that on the 30th and 60th day of the research the decrease of body mass gaining up to 10 % occurred, the decrease of protein and non-protein nitrogen content in liver and muscles in all the hens’ groups occurred. The increase of concentration of DNA and RNA phosphorus, the content of acid-soluble phosphate, phosphorus of phospholipid as well as lipoid phosphorus that indicates the slight deceleration of protein synthesis were determined, DNA and RNA in liver and hens’ muscles, also indicated the stimulation of synthesis of phosphorus acid ether and the reaction of glycolysis under the conditions of pesticide adding.