О методах сбора и препарирования жесткокрылых (Insecta: Coleoptera)

Abstract

The purpose of given article is observing of several problems of beetles collecting and preparation. The most of using collection methods are not correctly for ‘rare and little-known’ beetles. Rather in many collections beetles are mounted by an improper way. Incorrectly mounted and preparing materials frequently complicate work with them. Many structures appear deformed or latent from the researcher. Several methods of collecting and preparation partly based on authors and their colleagues’ personal experience are offered. The old museum materials, soften in distillated water with small quantity of alcohol (5–10 % solution) within day or boil in water about 4–10 minutes. It is not correctly to boil the beetles covered scales with metal shine, because they are loss the natural colouring. In a solution of alcohol or in water add 2–3 drops of a means for washing utensils. The beetles, killed by ethyl acetate are softened in water with several drops of alcohol during several hours. For a softening ‘formalin’ or ‘alcohol’ material use a solution of a vinegary acid or hot solutions of dairy or citric acids. As tools for dissection use various form and thickness preparing needle. It can be simply needles or needles with a hook on the end, or lancet form. For genitalia extraction separate abdomen from a body and boil the abdomen in 10 % solution kalium or natrium hydroxide in some minutes. It is convenient to make for this purpose water bath, which heat up on an electrical plate. Genitalia keep in glycerine in a tape ‘Korrex’ or in plastic test-tubes. For gluing it is better to use dry water-soluble glues.