Вивчення впливу нового похідного метилтеофіліну на функціональний стан нирок у щурів на тлі спонтанного діурезу

Abstract

Представлені результати вивчення впливу нового похідного метилтеофіліну на видільну функцію нирок у щурів в умовах спонтанного діурезу. 7-бензоілметил-8-(фуріл-2)-метиламінотеофілін в дозі 30 мг/кг за умов спонтанного діурезу стимулює фільтраційну функцію нирок, при тривалому застосуванні сприяє виділенню іонів натрію і меншою мірою — іонів калію.

The results concerning the impact of the new methyltheophylline derivative -7-benzoylmethyl-8-(furyl-2)-methylaminotheophylline on the activity of the kidneys in rats under conditions of spontaneous diuresis and figuring out some of the mechanisms of diuretic action for compounds. Research conducted on the white breed less rats of both sexes. Study of diuretic activity of 7-benzoylmethyl-8-(furyl-2)-methylaminotheophylline was carried out by E. B. Berchin. The content of electrolytes was determined using flame photometry, and an amount of creatinine formed by Folin’s method. The obtained data was calculated by techniques of nonparametric statistics using Student’s criterion (Lapach, Chubenko, & Babich, 2000). Based on the study of single and multiple (within seven days) introduction of 7-benzoylmethyl-8-(furyl-2)-methylaminotheophylline on the functional condition of kidneys in conditions of free access to the water and spontaneous diuresis, it was found, that single introduction of a dose of 30 mg/kg and under conditions of free access to water, 7-benzoylmethyl-8-(furyl-2)-methylaminotheophylline reduces drinking excitability to 2.4% (p<0.05), increases diuresis in 2.77 times (p<0.05), the concentration and excretion of sodium ions in 1.23 times (p < 0.05) and 3.05 times (p < 0.05), respectively; causes the growth of concentration and urinary excretion of potassium ions in 1.07 times (p < 0.05) and 2.68 (p < 0.05); increases the excretion of creatinine in 2.38 times (p<0.05) and slightly reduces the concentration of creatinine (7, 5%; p < 0.05). Observation of rats after applying of the 7-benzoylmethyl-8-(furyl-2)-methylaminotheophylline demonstrated, that on the 2nd day the indices almost returned to control values. Under conditions of multiple introduction of the studied compound the statistically reliable increase of urine output was observed. Average daily diuresis per seven-day period of 7-benzoylmethyl-8-(furyl-2)-methylaminotheophylline exceeded control period data in 2.77 times (p<0.05). After the cancelation of 7-benzoylmethyl-8-(furyl-2)-methylaminotheophylline the decrese in diuresis was noted. Reference drug hydrochlorothiazide in a dose of 25 mg/kg have spontaneous diuresis on only 70% (p<0.05), and the excretion of creatinine increased to 7.4% in comparison with control. Efficiency comparison of 7-benzoylmethyl-8-(furyl-2)-methylaminotheophylline with hydrochlorothiazide indicates a greater capacity of the last to excrete potassium (20.7%) and decrease urine sodium ions excretion (11.3%). It was determined, that in the conditions of spontaneous diuresis urination effected by 7-benzoylmethyl-8-(furyl-2)-methylaminotheophylline more definitely increases after reintroduction of it; prolonged application in the conditions of spontaneous diuresis, tested compound promotes the excretion of sodium ions and to a lesser extent — potassium ions.