Зміни біохімічних показників крові канарок кольорових за отруєння кантаксантином

Abstract

Встановлено, що токсикоз кантаксантином в кольорових канарок призводить до різких змін біохімічних показників крові даного виду тварин. Наведено ряд біохімічних показників крові канарок у нормі та їх зміни за дії кантаксантину.

Canthaxanthin (E-161g) is a nutritional supplement that is a physical disperse powder in red-orange color and belongs to a group of chemicals such as carotenoids. In the canal breeding, it is used as a support and amp red colored feathers. For cantaxanthin toxicity canaries can cause diarrhea, refusal of food, inhibition, shortness of breath, reduced reproductive ability; known cases of death of animals. In the literature, both domestic and foreign, almost no information about the pathogenesis, clinical and morphological diagnosis of cantaxanthin toxicosis. There is absolutely no data not only about changes in the biochemical parameters of blood canaries, but also about the normal values of these indicators. For the experiment, 20 color canaries were used at the age of 1.5 years, average fattening, normal physiological state. These birds were divided into 4 groups of 5 birds in each, three females and two males in each group. In the first group of experimental tubers fed corn mixes and soft feeds with addition of canthaxanthin in a dose of 5 g per 0.5 kg of feed or 0.5 l of water for 3 months. In the second group of experimental tubers fed corn mixes and soft feeds with the addition of canthaxanthin in a dose of 10 grams per 0.5 kg of feed or 0.5 l of water for 3 months. In the third group of experimental tubers fed corn mixes and soft fodders with the addition of canthaxanthin in a dose of 20 grams per 0.5 kg of feed or 0.5 l of water for 3 months. In the fourth (control) group of experimental tubers fed corn and soft food without the addition of canthaxanthin in the diet. The blood was selected in the area of the right cervical apteria from the right jaundice through an intravenous catheter with a diameter of 0.6 mm and placed in an epindorph. Blood tests were conducted on the following indicators: alanine aminotransferase (ALT), aspartate aminotransferase (AST), uric acid, total protein, glucose.The research was conducted on the basis of the veterinary laboratory of Bald Ltd., Kyiv. The resulting quantitative data was processed using the Microsoft Excel-2003 program. The study found that the use of canthaxanthin in a dose of 5 g did not increase the aminotransferase index. However, for a dose of 10 g in the second group, the ALT increased by 805%, in group 3 at a dose of 20 g by 1553%. ALT changes in animals in all experimental groups were bias. The AST index in the second group, as compared with that in the control animals, increased by 266%, and in the third group by 388%. Changes in AST in animals from the first and third groups were probable, and in animals of the second group, they were biased. An increase in the number of these enzymes indicates a toxic lesion of the liver, the presence of degenerative processes. Also, an increase in the uric acid content by 200% in the first and third groups and in 221% in the second experimental group, indicating excision of the renal excretory function and the probability of development of uric acid diathesis, was noted. Changes in the uric acid index in animals in all experimental groups were bias. The amount of total protein in animal experimental groups relative to this indicator in animals in the control group increased by 11-14%, which indicates the intoxication of the body, changes in the liver metabolism, and excitation of the excretory function of the kidneys. Changes in the total protein in animals in all experimental groups were probable for animal control in the control group. Changes in the amount of glucose in animals of the first and second experimental groups relative to such an indicator in animals of the control group were not noted, but in animals of the third group the glucose content was significantly increased by 17%, which may be a sign of liver and pancreas dysfunction.