Біохімічний склад крові корів за серозного маститу

Abstract

Нашими дослідженнями встановлено, що захворювання корів на серозний мастит супроводжується зниженням рівня загального білка в крові за рахунок зниження фракції альбумінів, значним зростанням фракції a - глобулінів та тенденцією до незначного зростання фракцій β- та γ – глобулінів. Кращий ефект при лікуванні корів з серозним маститом спостерігався в контрольній і III дослідній групах, про що свідчить зміна біохімічних показників.

Mastitis is one of the most significant and serious problems in dairy farming. This disease is still widespread throughout Ukraine among cows of different breeds. Currently, the understanding of only clinical, physiological and pathological indicators in diseases is not enough. It is also necessary to have data on biochemical changes in the body in any disease. It will help in deciphering the pathogenesis, detecting early diagnosis tests and testing the effectiveness of therapy methods. The purpose of our work was to study the dynamics of total protein and its fractions in the blood of clinically healthy and sick serous mastitis of cows using different methods of therapy for the further development of preventive measures of serous mastitis. The research was conducted in the farm of Sumy region in the summer-autumn and winter-spring periods of 2016-2018. For forming research groups of cows of the black-and-white breed with I and II calving with a diagnosis of serous mastitis are used, 15 animals of each were selected on the principle of analogues. A group of clinically healthy cows consisted of 10 animals. In the control group, a short novocaine blockade of the udder nerves was used according to D. Logvinov using 0.25% solution of novocaine at a dose of 150ml with the addition of 2 ml of hydrocortisone, three times, with an interval of 24 hours. In the first experimental group, an application was applied to the affected quarters of the udder of the emulsion, which included thiotriazolin, dimexide, menthol, anesthesin. The procedure was performed three times with an interval of 24 hours in combination with a light massage of the udder in the direction from the bottom up. In the second experimental group, a solution of 5 ml of thiotriazoline, 5 ml of 0.25% novocaine solution, 2 ml of dimexidum was injected into the affected quarter of the udder, three times, with an interval of 24 hours, in combination with a gentle massage of the udder. In the third experimental group, a complex treatment regime was used, which included thiotriazolin in a dose of 15 ml per cow in combination with the application of an emulsion consisting of dimexide, menthol, anesthesin, on the affected quarter of the udder, three times, with an interval of 24 hours. The content of total protein in serum was determined with biuret method, and protein fractions with electrophoresis. As a result of our research we determined that serous mastitis of cows is accompanied by a pronounced decrease in total protein in the blood due to a decrease in the albumin fraction, which is due to their easy penetration through the walls of blood vessels as a result of the inflammatory process, a significant increase in the fraction of α-globulins, which includes almost all proteins of the “acute phase”, and a tendency to a slight increase in the fractions of β- and γ-globulins, which shows at the intensification of immune processes in the body. The best effect of the treatment of cows with serous mastitis was observed in the control and III experimental groups, as evidenced by a change in biochemical parameters, in particular an increase in the level of total protein, albumin and γ-globulin, confirms the antiinflammatory, antihistamine, antioxidant, anesthetic, especially the immunostimulating effect drugs: thiotriazolin, novocaine, dimexidum and hydrocortisone.