Вплив різних титрів бактеріофагів на кількість Staphylococcus aureus variant bovis

Abstract

Наведено результати досліджень динаміки популяції фаг-бактерія при використанні різних титрів бактеріофага SAvB14 та чутливої до нього культури S. aureus var. bovis. Встановлено, що чим вищий титр фагів, тим інтенсивніше прогресує фагова інфекція. При цьому внесення фага з титром 10-8 БУО/см3 ефективніше знищує S. аureus, ніж внесення фаголізату з меншим вмістом SAvB14.

Nowadays bactheriophage treatment is more often seen as alternative to antibiotics. But collaborative mechanism of bacterial virus is not enough studied. The aim of work is to research the dynamic of bacterial virus population using different titres of bacteriophage SAvB14 and sensitive to its culture S. aureus var. bovis. To estimate the influence of bacteriophage Phage SAvB14 on amount of viable bacteria S. aureus var. bovis was taken 1 ml of phagolysate with titer of phagolysate with titers 10-6, 10-7, 10-8, 10-9BU/ml in 9 ml of nutrient broth with daily culture of tested microorganisms and kept during 12 hours. The amount of viable staphylococcus was defined every 2 hours by sowing them in the environment BD Baird-Parker Agar (HiMedia, India) according to standard methodic. The investigation was made three times. Strains S. aureus var. Bovis were used in the research, that were lysed by bacteriophage Phage SAvB14 in Otto method. The results of investigation show, that different titers of bacteriophage SAvB14 adversely affected on the target bacteria S. aureus during first two hours. However, the more titer of phage, the more progress phage infection. Staphylococcus content when interacting with phages, the titer of which 10-6BU / cm3 decreases in 1.6 times in12 hours of interaction, compared with the number of S. aureus var. bovis in broth without bacteriophage. The same results can be shown at a titer of 10-9 BU /cm3 - the content of S. aureus decreased in 2.3 times compared to the control. The obtained data indicate that the introduction of phage with a titer of 10-8BU / cm3 in 1.3 times more effectively destroys S. Aureus than the introduction of phagolysate with a titer of 10-7BU/cm3, and in 1.5 times compared with titer 10-6 BU/cm3. The results obtained from the research of the effect of phage with a titer of 10-9 BU/cm3, almost do not differ from obtained when testing a titer of 10-8BU/cm3. Therefore, to obtain desirable therapeutic effect in the use of phage drugs for the treatment of mastitis caused by S. aureus var. bovis, we recommend the use of phagolysate with a titer of at least 10-8BU / cm3.