Антиоксидантна і гепатопротекторна активність комбінації фосфатиділхоліну із сої і кверцетину

Abstract

У роботі наведені результати дослідження антиоксидантної і гепатопротекторної активності фосфатиділхоліну (ФХ) із сої, кверцетину і їх комбінації на моделі гострого токсичного ураження печінки щурів CCl4. Комбінація ФХ із сої і кверцетину проявляє високу антиоксидантну активність (на два – три порядка вище ФХ із сої і кверцетину в 1,7–2,0 рази) при ферментативному і аскорбатзалежному ПОЛ інтактних мікросом в системі in vitro і ефективніше ніж ФХ із сої і кверцетин окремо відновлює гідроксилазну активність і швидкість поглинання кисню при ферментативному ПОЛ мікросом в умовах гострого токсичного CCl4–гепатиту. Виражений терапевтичний ефект комбінації обумовлений мембранорепаруючими властивостями і антиоксидантними, посилюючою дією поліненасиченого ФХ із сої антиоксидантної дії кверцетину, що міститься у комбінації.

In the article the results of research of antioxidant and hepatoprotector activity of phosphatedilholin (PhH) from soya, quercetin and their combination on the model of acute toxic lesion of liver of rats CCl4 are determined. The results confirm that PhH combination from soya and quercetin shows high antioxidant activity (by 2-3 times higher from PhH from soya and higher of quercetin by 1,7–2,0 times) at enzymatic and ascorbate dependent POL in intact microsomes in the system in vitro. High antioxidant activity of combination can be explained due to the synergistic effect of quercetin and PhH from soya which combination contained. Higher antioxidant activity of quercetin compared to PhH from soya is caused by OH-groups and features of benzene ring of flavonoids which are included in its composition. In the conditions of experimental toxic hepatitis caused by CCl4 the speed of oxidative hydroxylation of amidopirin and consumption of oxygen at NADFH-dependent POL microsomes decreased that might be associated with the decrease of content of cytochrome P-450 and growth of speed of its inactivation, and also by the decline of content of phospholipids of membranes of microsomes. PhH combination from soya and quercetin, possibly to greater extent than PhH from soya and quercetin separately that were injected to the rats in doses 100mg/kg on the background of CCl4 pathology increased hydroxylase activity of microsomes compared to the animals with pathology. PhH combination from soya and quercetin also possibly to greater extent by 68,9% than PhH from soya by 54,1% and quercetin by 5,8% increased the speed of oxygen consumption at enzymatic POL microsomes compared to animals with pathology when at the same time the speed of oxygen consumption by microsomes at ascorbate dependent POL did not change under the influence of substances compared to intact control and also to animals with pathology. It should be noted that at introduction of PhH combination from soya and quercetin in a dose 100 mg/kg on the background of CCl4 pathology there was higher hydroxylase activity of microsomes compared to PhH from soya and quercetin that were injected separately. It correlated with increased speed of oxygen consumption at enzymatic POL of microsomes which confirms more intensive effect of PhH from soya which is in combination, in damaged CCl4 membrane of microsomes. Thus, PhH combination from soya and quercetin to greater extent showed a therapeutic effect than PhH from soya and quercetin injected separately that is caused by membrane reparative and antioxidant properties, by the strengthening effect of polyunsaturated PhH from soya, by antioxidant activity of quercetin which is in combination.