Спосіб корекції біохімічних змін в організмі бугаїв із гонадодистрофією токсичного типу (при хронічному нітратно-нітритному токсикозі)

Abstract

У статті представлені дані стосовно розробки способу корекції біохімічних змін при гонадодистрофії токсичного типу (при хронічному нітратно-нітритному токсикозі). Для корекції був використаний комплексний препарат «Карафанд+OV,Zn», що містить каротиноїди, фітоандрогени та наноматеріали – наночастинки ортованадату гадолінію активованого європієм та карбонату цинку. Після введення препарату спостерігали позитивну динаміку у прооксидантно-антиоксидантній системі, білково-вітамінно-мінеральному обміні та системі кисневого метаболізму. Даний спосіб корекції є простим у виконанні та виявляє високу терапевтичну ефективність, може бути рекомендованим до впровадження у практику ветеринарної репродуктології.

In the articles presented information in relation to development of method of correction of biochemical changes at gonadodystrophy of toxic type (at chronic to the nitrate-nitrite toxicosis). In the modern terms of conduct of plant-grower widespread is the use of nitric mineral fertilizers, here quantitative content of remaining matters often is uncontrolled, that is instrumental in the accumulation of nitrates in sterns. Use of such forage and water, muddy tailings of mineral fertilizers is a leading factor in etiology many illnesses of ruminant animals. The protracted use of such forage and water results in the origin of chronic toxicosis, in particular nitrate-nitrite. This type of toxins does the negative operating on the processes of exchange of matters in the organism of bulls and negatively influences on the reproductive system, assists development of gonadodystrophy. Previous researches, showed that at gonadodystrophy at terms chronic nitrate-nitrite toxicosis there are biochemical changes, above all things in to albumen-vitamin mineral exchange and prooxidant-antioxidant system. Task of research: to develop the method of correction of biochemical changes in the organism of bulls at gonadodystrophy of toxic type with the use of preparations, created on the basis of nano-biomaterials. Researches conducted on bulls (n=8) which belonged scientific and practical center of KSZVA, to some economies of the Kharkiv and Dnepropetrovsk areas, private individuals. Was experimental gonadodystrophy of toxic type caused orally with the feed of nitrate of sodium in a dose 0,3 grams NO3/kg the masses of body. Animals got complex preparation of «Karafand+OV,Zn», which contains carotenoids, phytoandrogens and nanomaterials of orthovanadate gadolinium activated europium and to the carbonate of zinc in a dose 3 ml for crawl and 20 ml for bull, orally, one time on days. Blood for an analysis was taken to introduction of preparation and on 20 days. A biochemical blood test was conducted in the Central research laboratory of the National pharmaceutical university. Amount of zinc was determined the method of atomic-adsorption spectrophotometry and conducted a chemiluminescent analysis in the laboratories of ISM NASU. The concentration of testosterone was determined in PI «Institute of problems of endocrine pathology the name of Danilevsky» with the use of method of linked immunosorbent assay (reagent kit of LTD RPC «GRANUM»). The method of correction of biochemical changes in the organism of bulls after gonadodystrophy of toxic type (at chronic to the nitrate-nitrite toxicosis) found out high therapeutic efficiency. A positive dynamics is marked in to albumen-vitamin mineral exchange, prooxidant-antioxidant system and oxygen metabolism. This method of correction is simple in implementation and finds out high therapeutic efficiency, can be recommended to introduction in practice of veterinary reproductology.