Морфологічні дослідження крові мишей за впливу мийно-дезінфікуючого засобу «Аргомол»

Abstract

У статті наведено результати досліджень впливу нового мийно-дезінфікуючого засобу «Аргомол» на морфологічні показники крові білих мишей. Експериментально доведено, що мийно-дезінфікуючий засіб, що досліджували, за введення його внутрішньошлунково об’ємом 1 см3 у 0,5 % концентрації, є безпечним для мишей та має невисоку токсичність.

The article presents the results of research of the influence of a new detergent and disinfectant «Argomol», created on the basis of lactic acid, «Katamine AB», polyhexamethyleneguanidine hydrochloride and colloidal silver solution on the morphological parameters of white mice blood. For the research a control and experimental group of white mice 10 heads each were formed. For animals of the experimental group the agent orally at 0.5 % concentration in an amount of 1 cm3 was applied. Subsequently, blood was collected for testing in 3 hours, 7 days, and 14 days after drinking the means and research were performed according to conventional methods. Initial hematologic parameters in the animals of the experimental and control groups were within normal limits. The total erythrocyte counts of both groups also remained within the physiological norm until the end of the research period. The white blood cell count of the experimental group 3 hours after introduction of detergent and disinfectant «Argomol» was significantly increased against the initial and control animals. Also, the mice in the experimental group responded to the disinfectant's allergic reaction, as evidenced by a significant increase in the number of eosinophils compared with controls and the original indicators. Hematological changes in the peripheral blood of white mice after introduction of 0.5 % detergent and disinfectant «Argomol» solution were temporary, as similar studies of peripheral blood 7 days after the application showed that all indicators, except for common leukocytes were within the physiological norm and remained the same until the end of the experiment. It has been experimentally demonstrated that detergent and disinfectant «Argomol», introducted intragastrically in a volume of 1 cm3 at 0.5 % concentration is safe for mice and has low toxicity. It was established that in animals of the experimental group 14 days after intragastrically introduction of 0.5 % of the concentrations of detergent and disinfectant «Argomol» which we developed all indicators of the functional state of peripheral blood were within the normal range.